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Year : 2014  |  Volume : 3  |  Issue : 1  |  Page : 33-39

Morphological, physico-chemical and structural characterization of mucilage isolated from the seeds of Buchanania lanzan Spreng

1 Department of Pharmaceutics, H.S.B.P.V.T.'s Group of Institutions, College of Pharmacy, Kashti, Ahmednagar, Maharashtra, India
2 Department of Pharmacology, Shree G. M. Bilakhia College of Pharmacy Rofel, Vapi, Gujarat, India

Correspondence Address:
Sudarshan Singh
H.S.B.P.V.T's Group of Institutions, College of Pharmacy, Kashti, Ahmednagar 414 701, Maharashtra
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2278-344X.130609

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Context: Mucilage isolated from the seeds of Buchanania lanzan a wild Indian plant. This mucilage can be commercially exploited, by evaluating physico-chemical properties of this mucilage. Materials and Methods: Various physico-chemical parameter using scanning electron microscopy (SEM), molecular weight, X-ray diffraction (XRD) spectrometry, zeta potential (ZP), Fourier transform infrared (FT-IR) spectroscopy and 1D ( 1 H and 13 C) nuclear magnetic resonance (NMR) have been employed to characterize mucilage in the present study. Results: SEM analysis suggests that the mucilage has irregular particle size. Thermogravimetry analysis suggested that mucilage had good thermal stability with two stage decomposition. The weight-average molecular weight of mucilage was determined to be 4883, by gel permeation chromatography. The XRD pattern of the mucilage indicated a completely amorphous structure. The ZP was obtained 1.56 and −9.49 mV in water and 0.1 N NaCl respectively. The major functional groups identified from FT-IR spectrum include 3459/cm (-OH), 1667/cm (Alkenyl C-H and C = C stretch), 1407/cm (-COO-) and 1321/cm (-CH 3 CO). Analysis of mucilage by paper chromatography and 1D NMR indicated the presence of rhamnose, arabinose and fructose. Conclusion: The spectral and chromatography analysis of mucilage indicate that the mucilage is composed of basic sugar moiety such as (arabinose, rhamnose, fructose and mannose) as complex carbohydrates.

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