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Year : 2017  |  Volume : 6  |  Issue : 1  |  Page : 11-14

A study on prevalence and antibiotic sensitivity testing methods for Neisseria gonorrhoeae isolates among female outpatients of sexually transmitted infection clinic

1 Department of Microbiology, Sri Venkateshwaraa Medical College Hospital and Research Centre, Puducherry, India
2 Department of Microbiology, Stanley Medical College, Chennai, Tamil Nadu, India

Correspondence Address:
Swapna Muthusamy
Department of Microbiology, Sri Venkateshwaraa Medical College Hospital and Research Centre, Ariyur, Puducherry - 605 102
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijhas.IJHAS_103_16

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CONTEXT: Gonorrhoea is the second most common sexually transmitted infection caused by the bacterium Neisseria gonorrhoeae. Though asymptomatic infections are common in females, adverse outcome is a long list and they serve as hidden sources for the transmission of infection. AIMS: To study the prevalence of N. gonorrhoeae infection among female outpatients and to compare Clinical Laboratory Standards Institute (CLSI) and calibrated dichotomous sensitivity (CDS) methods for antibiotic sensitivity testing. SETTINGS AND DESIGN: Cross-sectional study. SUBJECTS AND METHODS: Total number of subjects included in the study was 130. Two endocervical swabs were collected from each subject during speculum examination. Gram's stain, culture and antibiotic sensitivity test were performed as per CLSI and CDS methods. STATISTICAL ANALYSIS USED: Results were analysed by Chi-square test using IBM-SPSS Statistics-22 statistical package. RESULTS: Among the 130 females studied, three were positive for N. gonorrhoeae. Gram's stain and culture were equally sensitive. According to CLSI method, all the isolates were sensitive to spectinomycin, while other drugs showed 33% resistance. CDS method detected 33% resistance to ciprofloxacin and the rest of the antibiotics were 100% sensitive. CONCLUSIONS: Prevalence of N. gonorrhoeae was 2.3%. Gram's stain has got similar sensitivity as that of culture. CLSI method showed only spectinomycin was exclusively sensitive. CDS method showed all the tested drugs were sensitive except ciprofloxacin.

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