Molecular characterization of rotavirus genotype-A in children with acute diarrhea attending a tertiary hospital in Ilorin, Nigeria
Dele Ohinoyi Amadu1, Idris Nasir Abdullahi2, Anthony Uchenna Emeribe3, Peter Omale Musa4, Lawal Olayemi5, Thairu Yunusa6, Chisom Emmanuel Okechukwu7, Matthew Oluwafemi Salami8
1 Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria
2 Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, Zaria, Kaduna, Nigeria
3 Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria
4 Department of Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria
5 Department of Medicine, Faculty of Health Sciences, National University of Samoa, Samoa
6 Department of Medical Microbiology and Parasitology, University of Abuja, Abuja, Nigeria
7 Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
8 Department of Medical Microbiology, Federal School of Medical Laboratory Technology, Jos, Nigeria
Dr. Idris Nasir Abdullahi
Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, PMB 06 Shika, Zaria
Source of Support: None, Conflict of Interest: None
BACKGROUND: Despite indications that severe rotavirus diarrhea in children under <5 years of age is a major public health problem, only limited specific data on rotavirus burden are available in Sub-Saharan Africa. This study aimed to carry out molecular characterization of circulating rotavirus strains causing acute diarrhea among under-five children attending University of Ilorin Teaching Hospital (UITH), Ilorin, Nigeria.
MATERIALS AND METHODS: Ninety-three under-five children who are hospitalized with severe diarrhea were enrolled and their stool samples were collected for the detection and subsequent characterization for G and P rotavirus serotypes.
RESULTS: Of a total of 93 samples comprising 54 (58.1%) males and 39 (41.9%) females, 25 (26.9%) samples were positive for rotavirus by ELISA. Genotyping by RT-PCR was done on 25 samples. The most prevalent type-able VP7 G types were G9 (28%), G1 (24%), followed by G12 (20%), G2 (12%), and G10 (4%); on the other hand, the most prevalent type-able VP4 P types are P8 (48%), P4 (24%), and P6 (16%). The most common G-P combination was G9P (4) (20%) followed by G12P (8) and G1P (4) (16%, respectively); then GNTP (8) and G1P (6) (8%, respectively); and G9P (8), G12P (4), G10P (6), G2P (8), and G2P (6) (4%, respectively). The rotaviruses isolated from these children were from those <24 months of age (100%). There is statistical relationship between the prevalence of rotavirus infection with age (P = 0.033) but not with gender (P = 0.765).
CONCLUSION: This study highlights the rotavirus disease burden and diversity of rotavirus strains circulating in UITH. Continued sentinel surveillance will provide useful information to policy-makers with regard to rotavirus vaccine introduction.